![](https://parts.igem.org/images/partbypart/icon_regulatory.png)
Regulatory
Part:BBa_K1075001:Design
Designed by: Max Schelski Group: iGEM13_Bonn (2013-09-22)
Promoter(const.)-AraC-Term-pBAD (Arabinose inducable promoter system)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1234
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 1069
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 1051
Design Notes
the part was designed based on a sequence alignment of the expression plasmid pBAD with E. coli genomic sequence. Only the part matching the E. coli genome was amplified using specific primers.
Source
PCR amplification from an expression plasmid (pBAD) Whole sequence originally coming from E. coli genome as verified by BLAST