Regulatory

Part:BBa_K1075001:Design

Designed by: Max Schelski   Group: iGEM13_Bonn   (2013-09-22)


Promoter(const.)-AraC-Term-pBAD (Arabinose inducable promoter system)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1234
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1069
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 1051


Design Notes

the part was designed based on a sequence alignment of the expression plasmid pBAD with E. coli genomic sequence. Only the part matching the E. coli genome was amplified using specific primers.


Source

PCR amplification from an expression plasmid (pBAD) Whole sequence originally coming from E. coli genome as verified by BLAST

References